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Test Code TSPTQ T-SPOT.TB

Important Note

Order as a REF MISC test:

 

Laboratory Information and Test Link:

T-SPOT®.TB: https://testdirectory.questdiagnostics.com/test/test-detail/37737/t-spottb?cc=MASTER

 

Quest Diagnostics Nichols Institute 

14225 NewBrook Drive

Chantilly, VA 20153

Methodology

Enzyme-Linked Immunosorbent Assay

 

The T-SPOT.TB test is an in vitro diagnostic test for the detection of effector T cells that respond to stimulation by Mycobacterium tuberculosit antigens ESAT-6 and CFP 10 by capturing interferon gamma (IFN-y) in the vicinity of T cells in human whole blood collected in lithium heparin. it is intended for use as aid in the diagnosis of M. tuberculosis infection. The T-SPOT.TB test is an indirect test for M. tuberculosis infection (including disease) and is intended for use in conjunction with risk assessment, radiography and other medical and diagnostic evaluations.

Specimen Requirements

Container/Tube: 10 mL Green Lithium Heparin, Whole Blood (Quest Specialty Tube, LHMC Sendout Department Request)
Specimen Volume: 9 mL minimum volume
Collection Instructions:

 

  1. Collect blood by venipuncture
  2. Invert (8 - 10 times) all tubes to ensure that the whole blood is mixed thoroughly with the anticoagulant
  3. Do NOT centrifuge.

Day(s) Test Set Up

Sunday to Saturday

Reference Values

see laboratory report

Performing Laboratory

Quest Diagnostics Nichols Institute 

14225 NewBrook Drive

Chantilly, VA 20153

Test Classification and CPT Coding

86481

Specimen Requirements Additional Information

Specimen Stability:

Ambient: < 39 hours

Refrigerated: not acceptable

Frozen: not acceptable

Specimen Transport Temperature

  • Ambient YES
  • Refrigerate NO
  • Frozen NO