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Test Code TBQFT TB (QuantiFERON®) GOLD PLUS

Important Note

Order QuantiFERON-TB collection tubes using the Outpatient Laboratory Supply Order Form found under the FORMS header or contact the Logan Health Medical Center Laboratory at 406-752-1737.

Specimen must arrive within 14 hours of draw.

TB Quantiferon Collection Instructions

***Incubation period after receipt of specimen is 16 to 24 hours.

Methodology

In vitro diagnostic test using a peptide cocktail simulating ESAT-6, CFP-10, and TB7.7(p4) proteins to stimulate cells in heparinized whole blood. Detection of interferon-γ (IFN-γ) by enzyme-linked immunosorbent assay (ELISA) is used to identify in vitro responses to these peptide antigens that are associated with Mycobacterium tuberculosis infection.

 

This is an indirect test which is useful for detecting Mycobacterium tuberculosis infection (including disease). It is intended for use in conjunction with risk assessment, radiography, and other medical and diagnostic evaluations.

Specimen Requirements

Container/Tube: 4 TBQFT collection tubes (gray-top, green-top, Yellow-top and purple-top)
Specimen Volume: 4 mL of blood total (1 mL in each tube)
Collection Instructions:

  1. Draw blood in 4 special, TBQFT collection tubes (gray-top, green-top, yellow-top and purple-top). There is no order of draw for the four tubes.
  2. Allow each tube to fill completely to black mark (1 mL).
  3. Mix by firmly shaking the tubes up and down 10 times. 
  4. Do NOT centrifuge.

Day(s) Test Set Up

Monday, Wednesday and Friday

Reference Values

Negative

Performing Laboratory

Logan Health Medical Center Laboratory

Test Classification and CPT Coding

86480

Specimen Requirements Additional Information

Specimen Stability:

Ambient: 16 hours

Refrigerated: not acceptable

Frozen: not acceptable

Specimen Transport Temperature

Ambient/Refrigerate NO/Frozen NO