Test Code TBPCR MYCOBACTERIUM TB PCR
Methodology
Semi-quantitative nested real-time PCR
Useful for rapid detection of Mycobacterium tuberculosis complex DNA and Rifampicin resistance associated mutations of the rpoB gene.
Specimen Requirements
- Specimen source is required
- 24 hour collection is not acceptable
Submit only 1 of the following specimens:
Preferred:
Specimen Type: Expectorated sputum-24-Hour collection is
not acceptable.
Container/Tube: Sterile container, #6004913
Specimen Minimum Volume: 1 mL
Collection Instructions:
- Instruct patient to cough deeply and expectorate sputum into container.
- Label container with patient’s legal name, date of birth, date and time of collection, and type of specimen.
Note: A “deep cough” specimen, not saliva, must be collected for an adequate evaluation.
Alternate:
Specimen Type: Bronchial washing
Container/Tube: Sterile
container, #6004913
Specimen Volume: 1 mL to 2 mL
Collection Instructions: Label container with patient’s legal name, date of birth, date and time of collection, and type of specimen.
Alternate:
Specimen Type: Extra-pulmonary
Container/Tube: Sterile
container, #6004913
Specimen Volume: 1 mL to 2 mL
Collection Instructions: Label container with patient’s legal name, DOB, date and time of collection, and type of specimen.
Day(s) Test Set Up
Monday through Sunday
STAT Available 24/7
Maximum Laboratory Time
4 hours
Specimen Stability
Specimen Type | Temperature | Time |
Sputum | Ambient | < 3 days |
Refrigerated | 10 days | |
Frozen | NO |
Reference Values
Mycobacterium TB complex: Not detected
Rifampin: Not detected (when appropriate)
Critical Value (automatic call-back): <18 years - Detected
Performing Laboratory
Logan Health Medical Center Laboratory
Test Classification and CPT Coding
87556-MTB
87798-Rifampin (if appropriate)