Test Code SBPCR Streptococcus Group B, PCR
Methodology
Real-Time Polymerase Chain Reaction (PCR)/DNA Probe Hybridization
Testing Algorithm:
- If result is “Indeterminate,” a culture for group B streptococcus will be performed at additional charge.
- If result is “Detected” and patient is known to be allergic to penicillin, a culture and sensitivity for group B streptococcus will be performed at additional charge.
A qualitative test designed to detect Group B Streptococcus DNA in Lim Broth cultures, after 18 hours of incubation obtained from vaginal-rectal swabs. GBS bacterial infection is associated with serious illness in newborns born to women who are colonized with the microorganism. Transmission of GBS occurs from GBS-colonized women to their newborn before birth (antepartum) or during birth (intrapartum). In the United States, GBS infection is the major cause of death in newborns who develop sepsis, pneumonia, or meningitis.
Specimen Requirements
Vaginal/Rectal
Container/Tube: Eswab, White, #72732
Specimen Volume: Entire specimen
Collection Instructions:
- Wipe away excess vaginal secretion or discharge.
- Insert a large culture swab into lower one/third of vagina and sample secretions from mucosa.
- Carefully insert same culture swab approximately 1 inch beyond anal sphincter.
- Gently rotate swab to sample anal crypts. Feces should be evident on swab.
- Return swab to moist sponge in swab container to preserve specimen.
- Label container with patient’s legal name, date and actual time of collection, and type of specimen.
Maximum Laboratory Time
Monday through Friday, 36 hours
Specimen Stability
Ambient: 24 hours
Refrigerated: 6 days
Frozen: not acceptable
Reference Values
Not detected
Critical value (automatic call-back):
- <18 years - Detected
Performing Laboratory
Logan Health Medical Center Laboratory
Test Classification and CPT Coding
87150