Test Code NEOTX NEONATAL TRANSFUSION PROFILE
Methodology
Tube Testing and Gel System
- Testing includes a Type and Screen (TS) and Direct Antiglobulin Test (DAT).
- ABO & Rh, and DAT are performed on Baby's cord blood or peripheral blood specimen.
- Antibody screen, antibody identification (if needed), and crossmatch may be performed using Mom's plasma if available.
- All admissions to the Neonatal Intensive Care Unit will have a Neonatal Transfusion Profile performed.
- All NICU babies will be transfused with O Negative, CMV negative, Irradiated, freshest dated (<5 - 14 days old), red blood cells (CPD, CPDA-1 or AS-3).
Specimen Requirements
Submit only 1 of the following specimens:
- PREFERRED - Cord blood appropriately labeled
- PolicyStat – TM290 Blood Administration, Transfusion Management Policy, NICU < 4 months old considerations.)
- Alternative - 500 uL EDTA microtainer (lavender top) whole blood from the baby, appropriately labeled.
- From Mom - if available – 6 mL pink EDTA whole blood (preferred) or 4 mL EDTA lavender-top (alternate), appropriately labeled.
- (PolicyStat – TM290-Blood Administration, Transfusion Management Policy, NICU < 4 months old considerations.)
- NICU Baby specimens are kept for duration of admission and 14 days post hospital discharge.
- For specimens collected by a non-staff member or outside of the Logan Health System, the collector will not have mnemonics.
- At the discretion of the Blood Bank CLS, these can be accepted with Collector’s and Verifier’s; Initials, Printed Name, or Signature, as long as a location is specified.
- The patient name, date of birth, collection date, and collection time must be on the label.
Unacceptable
- Hemolyzed Specimens
- Mislabeled or unlabeled Specimens
- Illegible specimen labels - NO GEL PENS
Cord Blood
Nursing obtains Cord Blood and labels the tube at bedside with MobiLab or the baby's chart labels.
- Cord blood must be labeled with the following information:
- Baby's Name
- Baby's Birthdate
- Baby's account number – (If baby is a multiple i.e. twins)
- Mom's name
- Collector’s mnemonics and 2nd person Verifier’s mnemonics
- Collection date and time.
- Properly labeled Cord Blood is sent to the Lab.
Baby’s Peripheral Blood
- If no cord blood is obtained, a peripheral blood specimen will be collected from the baby and labeled as above.
Mom’s Blood (if Mom available to be drawn)
Check that the Mom is wearing a hospital identification wristband and baby identification wristband. Both bands must be attached to the Mom's body.
- Compare the Mom's wristband information to the baby identification wristband and with the information on the provider order.
- Write Mom’s name on Baby’s Lab labels for NEOTX using a ballpoint pen. (DO NOT USE GEL PEN).
- Have the Baby’s mother state their child's birthdate as a second method of identification.
- Use the Baby’s neonatal transfusion profile lab label or Baby’s Chart label to label Mom’s blood tube.
- The following information is required on the specimen tube:
- Baby’s identified name
- Baby’s Date of Birth
- Baby's account number – (If baby is a multiple i.e. twins)
- Notation , “Mom’s Blood”
- Collector’s mnemonics (and 2nd person Verifier mnemonics if not using MobiLab)
- Date and Time of Collection
- Properly labeled specimen is sent to the Lab.
Day(s) Test Set Up
Monday through Sunday
Reference Values
ABO: A, B, AB, or O
Rh: Positive or negative
Antibody Screen: Negative (if positive, an antibody identification will be performed.)
DAT: Negative
Performing Laboratory
Logan Health Medical Center Laboratory
Test Classification and CPT Coding
86850-Antibody screen
86900-ABO
86901-Rh
86880-DAT
86880-DAT. IgG (if appropriate)
86870-Antibody identification (if appropriate)
86903-Antigen identification per blood unit screened (if appropriate)
86922-Crossmatch, antiglobulin (if appropriate)
Specimen Transport Temperature
Refrigerate - 2 to 8 C
DAT must be performed within 24 hours of collection
Special Instructions
- Requisition - Use specific requisition from your EMR or Inpatient Department
- Blood Banking (Transfusion Medicine)—Blood Transfusion Policies and Standard Practices