Test Code MSI Microsatellite Instability Assay (Colorectal Cancer), PCR
Methodology
Multiplex Polymerase Chain Reaction (PCR) and Fragment AnalysisSpecimen Requirements
Submit only 1 of the following specimens:
Specimen Type: Formalin-fixed paraffin embedded (FFPE) tissue block-Tumor and normal tissue are required.
Container/Tube: FFPE of representative tumor tissue and representative normal colonic mucosa
Specimen Volume: One tissue block with representative tumor tissue and 1 tissue block with representative normal colonic mucosa
Alternate (if normal colonic tissue is unavailable):
Specimen Type: Peripheral whole blood
Container/Tube: Lavender-top (EDTA) tube
Specimen Volume: 1 mL (minimum volume: 0.5 mL)
Day(s) Test Set Up
Monday through SaturdayReference Values
An interpretive report will be providedPerforming Laboratory
US LabsTest Classification and CPT Coding
83891 x 2-Isolation or extraction of highly purified nucleic acid83900 x 2-Amplification, target, multiplex, first 2 nucleic acid sequences
83901 x 14-Amplification, target, multiplex, each additional nucleic acid sequence beyond 2
83907 x 2-Lysis of cells prior to nucleic acid extraction
83909 x 6-Separation and identification by high-resolution technique
83912-Interpretation and report