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Test Code MSI Microsatellite Instability Assay (Colorectal Cancer), PCR

Methodology

Multiplex Polymerase Chain Reaction (PCR) and Fragment Analysis

Specimen Requirements

Submit only 1 of the following specimens:

 

Specimen Type: Formalin-fixed paraffin embedded (FFPE) tissue block-Tumor and normal tissue are required.

Container/Tube: FFPE of representative tumor tissue and representative normal colonic mucosa

Specimen Volume: One tissue block with representative tumor tissue and 1 tissue block with representative normal colonic mucosa

 

Alternate (if normal colonic tissue is unavailable):

Specimen Type: Peripheral whole blood

Container/Tube: Lavender-top (EDTA) tube

Specimen Volume: 1 mL (minimum volume: 0.5 mL)

Day(s) Test Set Up

Monday through Saturday

Reference Values

An interpretive report will be provided

Performing Laboratory

US Labs

Test Classification and CPT Coding

83891 x 2-Isolation or extraction of highly purified nucleic acid

83900 x 2-Amplification, target, multiplex, first 2 nucleic acid sequences
83901 x 14-Amplification, target, multiplex, each additional nucleic acid sequence beyond 2
83907 x 2-Lysis of cells prior to nucleic acid extraction
83909 x 6-Separation and identification by high-resolution technique
83912-Interpretation and report

Specimen Transport Temperature

Refrigerate/Ambient NO/Frozen NO