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Test Code LPSBF Lipase, Body Fluid


Necessary Information


1. Date and time of collection are required.

2. Specimen source is required.



Specimen Required


Specimen Type: Body fluid

Preferred Sources:

-Peritoneal fluid (peritoneal, abdominal, ascites, paracentesis)

-Pleural fluid (pleural, chest, thoracentesis)

-Drain fluid (drainage, Jackson Pratt [JP] drain)

-Pericardial

Acceptable Source: Write in source name with source location (if appropriate)

Collection Container/Tube: Sterile container

Submission Container/Tube: Plastic vial

Specimen Volume: 1 mL

Collection Instructions:

1. Centrifuge to remove any cellular material and transfer into a plastic vial.

2. Indicate the specimen source and source location on label.


Useful For

Determining whether pancreatic inflammation or pancreatic fistula may be contributing to a pathological accumulation of fluid

Method Name

Enzymatic Colorimetric Reaction

Reporting Name

Lipase, BF

Specimen Type

Body Fluid

Specimen Minimum Volume

0.5 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Body Fluid Refrigerated (preferred) 7 days
  Frozen  30 days
  Ambient  24 hours

Reject Due To

Gross hemolysis Reject
Gross lipemia Reject
Gross icterus Reject
Cerebrospinal fluid, feces, breast milk, saliva, nasal secretions, gastric secretions, bronchoalveolar lavage (BAL) or bronchial washings, sputum, synovial, colostomy/ostomy, urine, or vitreous fluid Reject

Clinical Information

Lipases are enzymes that hydrolyze glycerol esters of long-chain fatty acids and produce fatty acids and 2-acylglycerol. The pancreas is the primary source of serum lipase. Pancreatic injury results in increased serum lipase levels. Serum lipase is measured to aid in the diagnosis of pancreatitis.

 

Peritoneal fluid:

The digestive enzymes amylase and lipase can be measured in the identification of pancreatic fluid in the peritoneal cavity. Concentrations are expected to be elevated and at least several-fold times higher in fluid of pancreatic origin compared to simultaneous concentrations in serum.(1,2)

 

Drain fluid:

Lipase is expected to be elevated in drain fluids formed due to chronic pancreatitis or formation of a fistula following surgery.(1,3,4) Comparison to serum concentrations is recommended with elevations several-fold higher than blood being suggestive of the presence of pancreatic fluid in the drained cavity.(5)

Reference Values

An interpretive report will be provided.

Interpretation

Fluids (peritoneal, drain):

Lipase concentrations several-fold higher than serum lipase concentrations is suggestive of the presence of pancreatic fluid in the drained cavity.

 

All other fluids:

Body fluid lipase activity may become elevated due to the presence of pancreatic fluid in the drained cavity. Results should be interpreted in conjunction with serum lipase and other clinical findings.

Cautions

In very rare cases of gammopathy, in particular type IgM (Waldenstrom macroglobulinemia) may cause unreliable results.

 

Calcium dobesilate causes artificially low lipase results.

Day(s) Performed

Monday through Sunday

Report Available

Same day/1 to 2 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test has been modified from the manufacturer's instructions. Its performance characteristics were determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information

83690

LOINC Code Information

Test ID Test Order Name Order LOINC Value
LPSBF Lipase, BF 15212-4

 

Result ID Test Result Name Result LOINC Value
LPBF Lipase, BF 15212-4
FLD7 Fluid Type, Lipase 14725-6