Test Code LACTIC.1 LACTIC ACID LEVEL
Methodology
Photometric, Lactate Oxidase/Peroxidase
Serum lactate is usually increased by strenuous muscular exercise or systemic infections. Lactic acidosis results from local tissue hypoxia caused by dehydration, poor perfusion, as a result of circulatory collapse, or cardiac failure. Elevated lactate levels are also seen in metabolic acidosis, diabetic ketoacidosis or nonketonic acidosis, type I glycogen storage disease, enzyme defects, alcoholism, neoplasia, and toxicity, including salicylate poisoning.
Specimen Requirements
Specimen must be centrifuged and plasma removed from cells within 15 minutes of draw. Blood should be drawn without the use of a tourniquet (or immediately after the tourniquet is applied).
Preferred:
Container/Tube: Grey-top (potassium oxalate/sodium fluoride) tube(s)
Specimen Volume: 1 mL (minimum volume: 0.5 mL) of plasma-Grossly hemolyzed specimen is not acceptable.
Collection Instructions: Fasting. Draw blood without using a tourniquet. Tube must be at least half-full. Place specimen on wet ice immediately.
Note: If there is a delay in transport of >15 minutes, spin down, remove plasma from cells, and send on wet ice.
Alternate:
Heparinized Plasma
Container/Tube: Green-top (lithium or sodium
heparin) tube(s)
Specimen Volume: 1 mL (minimum volume: 0.5 mL) of
plasma-Grossly hemolyzed specimen is not
acceptable.
Collection Instructions: Fasting. Draw blood without using a tourniquet. Tube must be at least half-full. Place specimen on wet ice immediately.
Note: If there is a delay in transport of >15 minutes, spin down, remove plasma from cells, and send on wet ice.
Day(s) Test Set Up
Monday through Sunday
Reference Values
0.7-2.1 mmol/L
Performing Laboratory
Logan Health Medical Center-Chemistry
Test Classification and CPT Coding
83605
Specimen Transport Temperature
On wet ice