Test Code KCHLG CHLAMYDIA PCR, GENITAL
Methodology
Polymerase Chain Reaction (PCR) Amplification and Hybridization Capture of Amplified Target.
(PCR is utilized pursuant to a license agreement with Roche Molecular Systems, Inc.)
Useful for detection of Chlamydia trachomatis from a swab or ThinPrep® PAP specimen.
Specimen Requirements
SUBMIT ONLY 1 OF THE FOLLOWING SPECIMENS:
Endocervix (Females Only)
Container/Tube: COBAS PCR Dual Swab Sample Kit #60546
Specimen Volume: Entire specimen
Collection Instructions:
- Use large woven sterile swab provided in collection kit to remove any excess mucus, blood, or examination lubricants.
- Insert the flocked sterile swab (brush) into endocervical canal. Rotate swab clockwise for 3 to 5 seconds and withdraw.
- Place swab in collection kit transport tube.
- Break swab shaft at score marking, replace cap tightly.
- Label tube with patient’s legal name, date and actual time of collection, and type of specimen.
Spermicidal agents and feminine powder sprays interfere with the assay and should NOT BE used prior to collection.
ThinPrep® PAP Media (Females)
Container/Tube: ThinPrep® preservative vial
Specimen Volume: Entire specimen on broom like device.
Collection Instructions:
- See “Cytopathology Specimen Collection and Handling, ThinPrep® Method” in “Cytopathology Specimen Collection and Handling”
- Label vial (not lid) with patient’s legal name, date of birth, collection date, clinician, and specimen source.
Throat
Container/Tube COBAS PCR Dual Swab Sample Kit #60546
Specimen Volume: Entire Specimen
Collection Instructions:
- Insert the large woven sterile swab into the pharynx and rotate against any inflammation and around the tonsillar area.
- Carefully removed the swab without touching any other area of the mouth.
- Place swab in collection kit transport tube.
- Break swab shaft at score marking, replace cap tightly.
- Label tube with patient’s legal name, date and actual time of collection, and type of specimen.
Male Urethra
Container/Tube: NP swab, #1178 (Green with thin flexible wire) in the COBAS PCR Dual Swab Sample Kit #60546, solution
Specimen Volume: Entire Specimen
Collection Instructions:
- Insert the NP swab into male urethra. Rotate swab clockwise for 2 to 3 seconds and carefully withdraw.
- Place swab into the Cobas (female PCR) collection kit transport tube.
- Cut nasopharyngeal swab shaft (wire), replace cap tightly.
- Label tube with patient’s legal name, date and actual time of collection, and type of specimen.
Rectum
Container/Tube: COBAS PCR Dual Swab Sample Kit #60546
Specimen Volume: Entire specimen
Collection Instructions:
- Insert the large woven sterile swab into the rectum approximately 4-6 cm and rotate against the rectal wall several times.
- Withdraw the swab carefully; avoid fecal contamination. If cross contaminated, discard and re-collect
- Place swab in collection kit transport tube.
- Break swab shaft at score marking, replace cap tightly.
- Label tube with patient’s legal name, date and actual time of collection, and type of specimen.
Specimen Retention Time
30 days
Day(s) Test Set Up
Monday through Friday
Batched Daily
STAT Available 24/7
Maximum Laboratory Time
72 to 96 hours
Specimen Stability
Endocervical Swab or Urethral Swab
Specimen Type | Temperature | Time |
Swab | Ambient | 1 hour |
Refrigerated | 7 days | |
Frozen | 30 days | |
ThinPrep® PAP Media
Specimen Type | Temperature | Time |
Thin Prep/PAP | Ambient | 6 weeks |
Refrigerated | 6 weeks | |
Frozen | NO | |
|
Reference Values
Not detected
Performing Laboratory
Logan Health Medical Center Laboratory
Test Classification and CPT Coding
Test Classification:
This test was developed and its performance characteristics determined by Logan Health Medical Center, Kalispell, MT. Use of this test for specimen source of ThinPrep® PAP media has been cleared or approved by the U.S. Food and Drug Administration.
CPT Code:
87491