Test Code INFSUBTYPE Influenza A Subtype (Post Pos Flu A)
Methodology
Reverse Transcription-Polymerase Chain Reaction (RT-PCR) / DNA
Probe Hybridization
(PCR is utilized pursuant to a license agreement with Roche
Molecular Systems, Inc.)
This test is useful as an aid in the differential diagnosis of
influenza A.
Includes the following subtypes of influenza A virus: seasonal H1, seasonal H3, and 2009 H1N1.
Specimen Requirements
Submit only 1 of the following specimens:
Preferred: Nasopharyngeal Swab
Container/Tube: Nasopharyngeal swab (Mini-Tip CULTURETTE®) placed in M4 or Universal Transport Media -Throat swab or calcium alginate or cotton-tipped swab is not acceptable.
Specimen Volume: Entire specimen
Collection Instructions:
1. Remove cap with swab attached. Holding swab between thumb and
forefinger of 1 hand and sheath in other hand, give soft wire a
10° to 20° bend. This slight curve fits nose better, making
procedure more comfortable for patient.
2. Slide swab along floor of the nose (hand down by patient’s
chin to start swab) with very light pressure to nasopharyngeal
wall.
3. Rotate swab and remove along floor of nose.
4. Return swab to M4 or Universal Transport Media to preserve
specimen.
5. Label container with patient’s legal name, date and actual
time of collection, and type of specimen.
Note: Specimen source is required.
Alternate: Nasal Wash
Specimen should arrive as soon as possible after
collection.
Container/Tube: Sterile vial(s)
Specimen Volume: >1 mL of wash material placed
in M4 or Universal Transport Media - Anterior nares
swab or excessively bloody specimen is not
acceptable.
Collection Instructions:
1. Immobilize patient in papoose board or wrap, or ensure
sufficient help to hold patient.
2. Lavage selected nare with 1 mL to 3 mL of saline.
3. Gently aspirate nare using a mucous trap with suction. Collect
wash material in trap vial and place in M4 or Universal Transport
Media.
4. Label vial with patient’s legal name, date, actual time of
collection, and type of specimen.
Note: Specimen source is required.
Day(s) Test Set Up
Monday through Friday
Reference Values
H1 seasonal influenza A not detected
H3 seasonal influenza A not detected
2009 H1N1 influenza A not detected
Performing Laboratory
Kalispell Regional Medical Center Laboratory
Test Classification and CPT Coding
87503x3-Infectious agent detection by nucleic acid (DNA or RNA); influenza virus, for multiple types or sub-types, multiplex reverse transcription and amplified probe technique, each additional influenza virus type or sub-type beyond 2
Specimen Requirements Additional Information
Specimen Stability:
Ambient: 2 to 8 hours
Refrigerated: 72 hours
Frozen: 30 days at -70° C.
Specimen Transport Temperature
Ambient