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Test Code HCVREFLEX HEPATITIS C AB WITH PCR REFLEX

Methodology

Chemiluminescent Immunoassay

If anti-HCV is positive,  Hepatitis C Virus (HCV) RNA Detection and Quantitation by Real-Time Reverse Transcription-PCR reflex testing (HCVQN) will be performed at an additional charge.  If HCV RNA levels are ≥ 500 IU/mL, HCV genotype testing (HCVGEN) will be performed at an additional charge.

Specimen Requirements

Specimen must be separated within 6 hours of the draw and refrigerated for < 24 hours.

 

Specimen Type: Serum-Plasma is not acceptable.

Container/Tube: Serum gel-Plain, red-top tube is not acceptable.

Specimen Volume (Preferred): 4.5 mL

Specimen Minimum Volume: 2.1 mL

Collection Instructions:

Spin down within 6 hours of draw.   If sample will not be received at LHMC Laboratory within 24 hours of collection, split serum sample and freeze.  Transfer serum into two separate aliquot tubes with each containing:

  • 1.0 mL (0.5 mL minimum)
  • 3.5 mL (1.6 mL minimum)

 

Day(s) Test Set Up

Monday through Friday  (HCV screen)

Specimen Stability

  • Refrigerate <24 hours
  • Frozen > 24 hours

Reference Values

Negative (reported as negative, indeterminate, or positive)

Performing Laboratory

Logan Health Medical Center-Chemistry

Test Classification and CPT Coding

86803-Anti-HCV
87521-HCV amplification (if appropriate)
87522-HCV RNA  (if appropriate)
87902-HCV genotype (if appropriate)