Test Code GTT3G GLUCOSE TOLERANCE 3 HOUR, GEST
Methodology
Colorimetric
Useful for diagnosing glucose intolerance in pregnant women at high-risk for gestational diabetes and for confirmation of abnormal GLU.CHAL “Glucose Challenge, Plasma or Serum” when performed at 24 to 28 weeks of pregnancy.
Specimen Requirements
Patient Preparation:
- Patient must be on a normal diet for 3 days prior to testing
- Fasting for at least 8 to 14 hours prior to test
Procedure:
See “Glucose Tolerance Testing, Gestational” in Special Instructions for dosage and collection times.
Specimen Collection:
Submit only 1 of the following specimens for each draw time:
Preferred:
Plasma
Container/Tube: Green-top (lithium heparin) tube(s) or grey-top (potassium oxalate/sodium fluoride) tube(s)
Specimen Volume: 1 mL (minimum volume: 0.5 mL) of plasma-Grossly hemolyzed specimen is not acceptable.
Collection Instructions: Spin down within 1 hour of draw.
Note:
- Grey-top tube is stable for 24 hours after drawing specimen.
- Label specimens appropriately (time of draw and as plasma).
Alternate:
Serum
Serum Gel Tube
Container/Tube: Serum gel tube(s)
Specimen Volume: 1 mL (minimum volume: 0.5 mL) of serum-Grossly hemolyzed specimen is not acceptable.
Collection Instructions: Spin down within 1 hour of draw.
Note: Label specimens appropriately (time of draw and as serum).
Alternate:
Serum
Plain, Red-Top Tube
Container/Tube: Plain, red-top tube(s)
Specimen Volume: 1 mL (minimum volume: 0.5 mL) of serum-Grossly hemolyzed specimen is not acceptable.
Collection Instructions: Spin down within 1 hour of draw.
Note: Label specimens appropriately (time of draw and as serum).
Day(s) Test Set Up
Monday through Sunday
Reference Values
Fasting: <95 mg/dL
1 hour: <180 mg/dL
2 hour: <155 mg/dL
3 hour: <140 mg/dL
Diagnosis of gestational diabetes requires that 2 or more of the above limits are met or exceeded.
Critical values (automatic call-back): <50 mg/dL or >500 mg/dL
Performing Laboratory
Logan Health Medical Center-Chemistry
Test Classification and CPT Coding
82951-Glucose tolerance test, 3 specimens
82952-One additional specimen (if appropriate)
Specimen Transport Temperature
Refrigerate