Sign in →

Test Code DLL3 Delta-Like 3 Protein (SP347), Semi-Quantitative Immunohistochemistry, Manual, Tissue


Shipping Instructions


Attach the green "Attention Pathology" address label (T498) and the pink Immunostain Technical Only label included in the kit to the outside of the transport container.



Necessary Information


A pathology/diagnostic report and a brief history are required.



Specimen Required


Specimen Type: Tissue

Supplies:

-Pathology Packaging Kit (T554)

-Immunostain Technical Only Envelope (T693)

Container/Tube: Immunostain Technical Only Envelope

Collection Instructions: Formalin-fixed, paraffin-embedded tissue block; or 3 unstained glass, “positively charged” slides with 4-microns, formalin-fixed, paraffin-embedded tissue

Additional Information: One slide will be stained with hematoxylin and eosin and returned.


Useful For

Diagnosis of small cell lung carcinoma (SCLC), large cell neuroendocrine carcinoma (LCNEC), amongst other tumors

Method Name

Immunohistochemistry (IHC)

Reporting Name

DLL3(SP347)Semi-Quant IHC,Manual

Specimen Type

Special

Specimen Stability Information

Specimen Type Temperature Time Special Container
Special Ambient (preferred)
  Refrigerated 

Reject Due To

Wet/frozen tissue
Cytology smears
Nonformalin fixed tissue
Nonparaffin embedded tissue
Noncharged slides
ProbeOn slides		 Reject
 

Clinical Information

Delta-like 3 protein (DLL3) is an atypical Notch ligand induced by the neuroendocrine transcription factor, ASCL-1.  DLL3 is expressed on the cell membrane and in the cytoplasm of tumor cells. 

Interpretation

Positivity for this test will require 50% or more of the tumor cells within a specimen to express delta-like 3 protein with an intensity ranging from 1 to 3+.

Cautions

This test has been validated for non-decalcified paraffin embedded tissue specimens fixed in 10% neutral buffered formalin. This assay has not been validated on tissues subjected to the decalcification process and/or use of alternative fixatives for bone/bone marrow specimens or cell blocks.

 

Age of a cut paraffin section can affect immunoreactivity. Stability thresholds vary widely among published literature and are antigen dependent. Best practice is for paraffin sections to be cut within 6 weeks.

Day(s) Performed

Monday through Friday

Report Available

5 to 7 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information

88360

LOINC Code Information

Test ID Test Order Name Order LOINC Value
DLL3 DLL3(SP347)Semi-Quant IHC,Manual In Process

 

Result ID Test Result Name Result LOINC Value
603333 Interpretation 59465-5
603334 Participated in the Interpretation No LOINC Needed
603335 Report electronically signed by 19139-5
603336 Material Received 81178-6
603337 Disclaimer 62364-5
603338 Case Number 80398-1

Forms

If not ordering electronically, complete, print, and send a Immunohistochemical (IHC)/In Situ Hybridization (ISH) Stains Request (T763) with the specimen.