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Test Code DAT.1 (Direct Antiglobulin Test) DIRECT COOMBS  

Methodology

Tube Testing

The Direct Antiglobulin Test (DAT) is useful for detecting antibodies and complement adsorbed to the surfaces of red blood cells in the body. This test is mainly used to investigate autoimmune hemolytic anemia (AIHA), hemolytic disease of the newborn, drug induced hemolysis, and alloimmune reactions to recently transfused cells.

Specimen Requirements

Specimen must arrive within 24 hours of draw.

 

** Referal DAT can be accepted if specimen is > 24 hours old, but < 72 hours old. These will be resulted with a disclaimer. 

 

Container/Tube: Pink-top (EDTA) “crossmatch” tube(s) or pink-top (EDTA) MICROTAINER®-Plain, red-top tube or serum gel tube is not acceptable.

 
Specimen Volume: 6 mL to 7 mL (minimum volume: 0.5 mL/pediatric: 0.5 mL in EDTA MICROTAINER®) of whole blood

 

Collection Instructions: Forward promptly in original tube(s)/MICROTAINER®.

Day(s) Test Set Up

Monday through Sunday

Reference Values

Negative

Performing Laboratory

Logan Health Medical Center Laboratory

Test Classification and CPT Coding

86880-IgG

86880-C3b, C3d

Specimen Transport Temperature

Refrigerated