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Test Code 00025 Sentinel Node Biopsy, Lymph Node

Methodology

A sentinel lymph node is the “first” node in a lymphatic drainage basin, and is evaluated for metastasis from the original cancer (breast or melanoma). The tumor excision usually accompanies the sentinel nodes.

Specimen Requirements

Container/Tube: Sterile container
Specimen Volume: Entire specimen


Collection Instructions:

The Surgery Department must retain all specimens (with adequate formalin added) from a sentinel node recovery procedure encased in lead for 24 hours. Excised breast and axillary tissue (in the case of a mastectomy/lumpectomy) or wide excision melanoma specimen should also remain under lead with their respective sentinel nodes for 24 hours. 

 

After 24 hours, specimens should be transported to the Pathology Department:

  1. The ratio of tissue to fixative is important. In general, a specimen requires about 5 to 15 times its volume of fixative to allow adequate fixation. 
  2. Tissues that are not placed in fixative or that are put into inadequate volume of fixative may undergo autolysis and be irretrievably damaged.

 

Note:

1. Label container with patient’s legal name, date of birth, tissue source, and physician’s name.

2. Specimen source, clinical history/diagnosis, and physician’s name are required.

 

Forms: “Pathology Requisition” (Listed in Special Instructions)

 

Day(s) Test Set Up

Monday through Friday

Specimen Stability

Ambient

Reference Values

An interpretive report will be provided.

Appropriate stain(s) will be performed and charged separately.

Performing Laboratory

Logan Health Medical Center Laboratory

Test Classification and CPT Coding

Varies

Special Instructions

Note: Complete ALL information including specimen source and all pertinent clinical data (clinical symptoms, history of malignancy, chemotherapy, radiation therapy, and tentative diagnosis) and forward with specimen.